5-Aminolevulinic Acid Dehydratase. The Role of Sulphydryl Groups in 5-Aminolevulinic Acid Dehydratase from Bovine Liver

-Aminolevulinic Acid Dehydratase from Plasmodium falciparum

The heme biosynthetic pathway of the malaria parasite is a drug target and the import of host -aminolevulinate dehydratase (ALAD), the second enzyme of the pathway, from the red cell cytoplasm by the intra erythrocytic malaria parasite has been demonstrated earlier in this laboratory. In this study, ALAD encoded by the Plasmodium falciparum genome (PfALAD) has been cloned, the protein overexpre...

Molecular cloning of the 5-aminolevulinic acid dehydratase gene from Rhodobacter sphaeroides.

A hemB mutant of Escherichia coli was used to clone the gene encoding 5-aminolevulinic acid dehydratase from Rhodobacter sphaeroides after physiological complementation of the mutation. A 2.9-kb DNA fragment was obtained and cloned in both orientations into the unique PstI restriction site of pUC19. This recombinant plasmid encodes a protein (Mr 39,000) that is immunoreactive with antibodies ra...

A cDNA clone for 5-aminolevulinic acid dehydratase from tomato (Lycopersicon esculentum Mill.).

Evaluation of Erythrocyte 5-Aminolevulinic Acid Dehydratase Activity in the Blood of Crucian Carp, Curassius

The activity of 5-aminolevulinic acid dehydratase (ALA-D) in the blood of crucian carp, Carassius auratus langsdorfiii, was measured under a variety of lead exposure conditions. A three-week exposure of crucian carp to water lead concentrations of 3, 10, and 30 ppb inhibited their ALA-D activities with increasing water lead concentrations. When crucian carp were exposed to a water lead concentr...

Induction of 6 - Aminolevulinic Acid Dehydratase in Mouse Friend Virus

The activity of S-aminolevulinic acid (ALA) dehydratase. an enzyme involved in heme biosynthesis. has been shown to increase in Friend virus-transformed murine erythroleukemia (MEL) cells during erythroid differentiation. In this study. the nature of the increase in ALA dehydratase activity in MEL cells was examined using a monospecific antibody directed to the enzyme. A sevenfold increase in A...